The Antibody Stains CD8+ T Cells Very Well

May 03, 2017

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Brilliant Violet 421™ anti-mouse CD8a Antibody

Catalog Number:

100753

We wanted to study T cells in diseased vs healthy states, so we used this antibody to identify the CD8+ T cell subset.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of mouse lymph node cells

Primary Incubation

1:100 in staining buffer for 25 minutes at 4 degrees

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody clearly stains CD8+ T cells very well

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

The antibody stains CD8a+ T cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for clearly staining CD8a+ T cells

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