Abcam
Anti-TOMM20 antibody - Mitochondrial Marker
ab78547
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This antibody (ab78547) was used to perform immunofluorescence microscopy on HeLa cells to detect endogenous TOMM20 in the mitochondria. ab787547 showed strong immunoreactivity that was consistent mitochondria. Background levels were low and the staining was not as clear as mitotracker from Thermofisher. This antibody is a good choice for multicolor microscopy.
Immunofluorescence
HeLa cells fixed with 3.7% paraformaldehyde
1 hour at room temperature in blocking agent at 1:300
PBS in 1% normal goat serum and 0.1% triton x-100
Goat anti-rabbit conjugated to Alexa 568 for 1 hour at room temperature (1:300)
DAPI
Confocal microscopy
This antibody showed good results in HeLa cells. The staining was not as robust as it is with mitotracker, but the staining was consistent with mitochondria.
N/A
Good rabbit antibody for TOMM20
The quality is not as good as with mitotracker from Thermo Fisher Scientific
A good choice that works on fixed HeLa cells.