CD56 Microbeads For Purification Of Human NK Cells

Albert Einstein College of Medicine
Genetics
Post Doc

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Miltenyi Biotec

Product Name:

CD56 MicroBeads, human

Catalog Number:

130-050-401

Our laboratory investigates different ways to improve immune responses against HIV, including NK mediated ADCC. For our in vitro and in vivo ADCC assays we need to isolate or deplete NK cells from human PBMCs, for which we use these beads.

Experimental Design and Results Summary

Application

Cell purification

Starting Material

Human PBMCs

Protocol Overview

Resuspend cells in 80ul of buffer per 10*10^7 and add 20ul of beads. Incubate the on ice for 15min. wash the cells and proceed to magnetic separation.

Tips

Follow the manufacturers protocol

Results Summary

Good separation of CD56+ cells from human PBMCs.

DOI or PMID #

N/A

Additional Notes

N/A

Image Gallery

Summary

The Good

Consistent results, easy to use.

The Bad

None

The Bottom Line

Great product for human CD56+ cells purification.

Join the discussion