Akt3 Rabbit mAb For Western Blot

Pathology
Albert Einstein College of Medicine
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Cell Signaling Technology

Product Name:

Akt3 (E2B6R) Rabbit mAb

Catalog Number:

14293

Our lab studies the role of Akt3 in various models of multiple sclerosis including EAE and cuprizone. To assess Akt3 protein levels in various tissues and cell types, we have employed a number of available Akt3 antibodies. The E2B6R clone works great in western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse brain homogenate

Primary Incubation

Akt3 (E2B6R) Rabbit mAb

Blocking Agent

5% milk 5% goat serum

Secondary Incubation

LI-COR anti-mouse IRDye secondary antibody

Tertiary Incubation

None

Detection

LI-COR Odyssey

Results Summary

Western blots appear clean with little to no background and single band at the expected band size of ~60kDa.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Detects a band at the appropriate band size

The Bad

Rabbit "mAb"...if such a thing actually exists.

The Bottom Line

Good antibody for western blot detection of Akt3

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