Submandibular Blood Sample Collection

Albert Einstein College of Medicine
Pathology
Research Fellow

Overall

Performance

Ease-of-Optimization

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Company:

Medipoint

Product Name:

Goldenrod Animal Lancet

Catalog Number:

Goldenrod 5mm

We wanted to collect multiple blood samples quickly and safely in one day, without causing harm to the animals (mice). I used these lancets to collect small amounts of blood (200ul) from the cheek of mice without using anesthesia. We used these lancets as opposed to eye bleeding because we wanted to avoid blindness in our mice and the use of multiple doses of anesthesia in one day. Our end goal is to determine the levels of a specific antibody in the blood and it's affect on paralysis.

Experimental Design and Results Summary

Application

Blood Collection for ELISA

Starting Material

Mice and Lancet

Protocol Overview

With the mouse secured in one hand. The lancet was used to quickly inject the submandibular located at the rear of the jaw bone. The blood serum was then collected and processed for ELISA

Tips

Be sure not to strangle the mice when holding them. Ensure that they are breathing before using the lancet.

Results Summary

We were able to collect enough blood serum from each mouse twice a day in order to measure protein levels by ELISA.

Features Summary

A great way to draw blood

DOI or PMID #

N/A

Additional Notes

N/A

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Summary

The Good

Quick recovery and fast

The Bad

Securing mice can be challanging, practise before performing on essential mice

The Bottom Line

If you need to collect blood samples multiple times a day. This is the best option, especially for mice.

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