Great Mouse Anti-Tubulin Antibody

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate Student

Overall

Quality of Results

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Company:

Sigma Aldrich

Product Name:

Monoclonal Anti-α-Tubulin antibody produced in mouse clone DM1A

Catalog Number:

T9026

Browse Similar Products: Tubulin Immunofluorescence Antibodies

We use this antibody to identify microtubules in cells. This antibody gives a strong, crisp pattern with limited background.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

PFA Fixed U2OS cells

Primary Incubation

1:500 at room temperature for 1.5 hours

Blocking Agent

5% NGS

Secondary Incubation

1:750 CY2 conjugated anti-mouse antibody for 1 hour at room temperature

Tertiary Incubation

None

Detection

Confocal Microscope

Results Summary

Microtubule antibody very sharply binds to the array within the cell. There is minimal background and we can use this staining to determine array organization.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Crisp, clear staining at a low antibody concentration.

The Bad

None. Clean.

The Bottom Line

Great, highly recommended tubulin antibody.

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