ThermoFisher/eBioscience
Anti-Human IgE APC
17-6986-42
Info: View Product View Product Specs
Browse Similar Products: IgE Flow Cytometry Antibodies
IgE antibodies are integral to allergic reactions. We sought to measure cell-bound IgE on peripheral blood mononuclear cells in healthy and allergic subjects. We used this antibody because it recognizes IgE both in solution and when bound to cellular receptors (i.e., the epitope is not obscured).
Flow Cytometry
Human peripheral blood
1.25ug/ml (5ul of antibody for 100ul blood) for 30min at room temperature
FcX TruStain (Biolegend, 422302), 5ul
None
Flow cytometry, APC channel on LSR Fortessa
Bright staining on basophils (CCR3+), which express the high affinity IgE receptor (FceRI). Minimal background on B cells (CD19+) or T cells (CD3+). Used at the recommended concentration, the antibody produced brighter staining on basophils from allergic subjects (who have higher IgE levels). Used at a 5x lower concentration (1ul), staining was adequate to identify basophils, but showed a reduced ability to distinguish subjects with high vs. low IgE levels.
Bright, easily titrated, minimal background
Needs to be used as directed, at high amounts for best results
Bright, works well, stains as expected.