Cell Signaling Technology
Phospho-VEGF Receptor 2 (Tyr1175)
2478
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Activated VEGFR2 can be detected by the phosphorylation of tyrosine residue 1175. This can be detected by western blot using this antibody
Western Blot
HUVEC lysate
1:2000, overnight
5% BSA
1:5000, 1 hour
NA
ChemiDoc™ Touch Imaging System
Bands were detected at the correct size however blots were fairly dirty and so quantification is difficult.
Can pick up pVEGFR2
Dirty blot
Good to detect activation of VEGFR2, however not great to quantify level of activation