Actin Antibody For Western Blot

March 08, 2017

Physiology and Biophysics
Albert Einstein College of Medicine
Graduate student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Abcam

Product Name:

Anti-actin antibody (AC40)

Catalog Number:

ab11003

Actin is widely used as a loading control for Western blots. This antibody is reliable and give clean results.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HUVEC lysate

Primary Incubation

1:2000, overnight

Blocking Agent

5% milk

Secondary Incubation

1:5000, 1 hour

Tertiary Incubation

NA

Detection

ChemiDoc™ Touch Imaging System

Results Summary

Clean bands were detected at the expected size reported for actin

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Clean bands

The Bad

Overnight incubation

The Bottom Line

A great antibody to use to detect actin in western blot experiments

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