Invitrogen by Thermo Fisher Scientific
anti-v5 antibody
46-0705
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Different cyclic-di-peptides were used to test if it could affect the expression of cereblon in HCT116 cells. HCT116 were transfected with the V5-CRBN plasmids using lipofectamine 2000. The expression of V5 was examined by western blotting.
Western Blot
HCT116
1/5000
5% milk
1/10000
1 hour at room temperature
ECL
The CRBN tranfecton was successful as indicated from the blotting results
N/A
It works perfectly
The system works