The Good
Simple protocol, relatively easy to optimize.
The Bad
Make sure to titrate/optimize antibody with your sample type prior to use on precious samples/materials.
The Bottom Line
I recommend this product for use in flow cytometry assays designed to identify and quantify sub-populations of T-cells from human whole blood samples. When combined, the color combinations (see my other reviews for this protocol) included in the T-cell panel provide clean, consistent results.