Anti-Human CD16 Mouse Monoclonal Antibody For IHC

February 08, 2017

Clinical Research
Albert Einstein College of Medicine
Pre-doctoral researcher

Overall

Quality of Results

Ease-of-Optimization

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Company:

Leica Biosystems

Product Name:

Anti-human CD16 mouse monoclonal antibody

Catalog Number:

NCL-L-CD16

Browse Similar Products: CD16 Immunohistochemistry Antibodies

We used Leica's anti-human CD16 monoclonal antibody to stain our paraffin embedded brain sections for monocytes. The antibody was recommended to us by a collaborator and after easy optimization (including the appropriate dilution and incubation time) we decided to continue using the antibody for all of our IHC experiments. The antibody is suggested for use specifically with paraffin sections, and the catalog number above corresponds to the reagent in a liquid format (as opposed to a lyophilized format).

Experimental Design and Results Summary

Applications

Immunohistochemistry

Sample

Paraffin embedded section of a human specimen (i.e. tissues)

Primary Incubation

1:10 dilution in 10% NHS at 4 degrees C overnight (cold room)

Blocking Agent

NHS (normal horse serum)

Secondary Incubation

ImmPRESS Reagent Kit (anti-mouse Ig) at room temperature for 30 minutes

Tertiary Incubation

No tertiary antibody/incubation

Detection

DAB+ Substrate Kit -- developed at room temperature for 6 minutes

Results Summary

Per the manufacturer's guidelines, successful staining should be determined by light microscopy in the presence of the appropriate controls (with known staining patterns).

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Relatively inexpensive, easy to optimize, reliable and consistent results.

The Bad

Not sold by many outside retailers -- must contact company for quote.

The Bottom Line

I would recommend this product to individuals staining human paraffin embedded sections for immune cells. The antibody is specific, and once the staining protocol is optimized for the tissue/cells of interest, the results are consistent and reliable.

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