Product Review For Anti-Human CXCR7 Antibody By Biologend

Cell and Molecular Biology
Northwestern University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Anti-human CXCR7

Catalog Number:

391402

The overall goal of this study is to study transmission of HIV/SIV via sexual mode. We specifically wanted to phenotype the cells infected by staining for variety of chemokine receptors in various macaque tissues. We choose this antibody since it was suggested that it works in our specie of macaques (rhesus) for the purposes of fluorescent microscopy.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Rhesus Macaque Tissue

Primary Incubation

One hour at room temperature at 1:100 dilution in PBS

Blocking Agent

Blocking buffer (Donkey Nomral Serum)

Secondary Incubation

30 minutes at room temperature AF647 antibody at 1:800 dilution in PBS

Tertiary Incubation

None

Detection

Fluorescence Microscopy

Results Summary

This antibody works well in anorectal tissues of Rhesus Macaque. No background problems or non specific binding was encountered.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

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Summary

The Good

Very quick incubation protocol

The Bad

Not directly conjugated

The Bottom Line

I would recommend this product for use in Rhesus tissues.

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