CD38 Monoclonal Antibody (HIT2), APC.

January 17, 2017

Biomedical Sciences
Cornell University
Research Associate

Overall

Quality of Results

Ease-of-Optimization

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Company:

Invitrogen

Product Name:

CD38 Monoclonal Antibody (HIT2), APC

Catalog Number:

MHCD3805

HL-60 cells are able to differentiate to neutrophils after all trans retinoic acid (RA) treatment. They are also able to differentiate to monocytes after 1,25-dihydroxyvitamin D3 (D3) treatment. A characteristic of this conversion is CD38 expression. Cell culture volumes containing one million cells were spun down and resuspended in 200µl PBS with 2.5µl CD38 Invitrogen MHCD3805 Lot 1662185A antibody. This antibody has very low background but is expensive.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

HL-60 cells ; untreated, all trans retinoic acid (RA), 1,25-dihydroxyvitamin D3 (D3) treated

Primary Incubation

1 million cells were incubated 1h , 37C, in 200µl PBS with 2.5µl CD38 antibody

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Instrument: BD LSR II. APC was excited at 633 nm and emission was collected through 660/20 band-pass filter.

Results Summary

CD38 Monoclonal Antibody (HIT2), APC. Catalog#. MHCD3805 Lot 1662185A has very low background. The cost is $1.55/ sample.

DOI or PMID #

10.1016/j.fob.2015.04.002.

Additional Notes

N/A

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Summary

The Good

CD38 Monoclonal Antibody (HIT2), APC. Catalog#. MHCD3805 Lot 1662185A has very low background. The review is for Lot 1662185A, however this catalog number had consistent quality over many years.

The Bad

Expensive.

The Bottom Line

A very good antibody, but expensive.

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