Expression of Survivin in HEK293 Cells

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

Overall

Quality of Results

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Company:

Cell Signaling Technology

Product Name:

Anti-Survivin Antibody

Catalog Number:

2808

Survivin is a member of the inhibitor of apoptosis (IAP) family and it functions to inhibit caspase activation which leads to negative regulation of apoptosis or programmed cell death. It has been shown that disruption of survivin induction pathways leading to increase in apoptosis and decrease in tumour growth. The present study aims to check the expression of Survivin in HEK293 cells by western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

HEK293 cells

Primary Incubation

1:1000

Blocking Agent

No blocking

Secondary Incubation

1:10000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody (CST#2808) detected Survivin at around 16 kDA in HEK293 cells by western blot.

DOI or PMID #

NA

Additional Notes

NA

Related Categories

Image Gallery

Summary

The Good

Identifies clear band at right size

The Bad

Nothing

The Bottom Line

Good antibody to detect survivin protein

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