Bosterbio
EK0309 Mouse BDNF PicoKine™ ELISA Kit
EK0309
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I used the ELISA kit to detect the BDNF level in several samples, including brain tissues (cortex and hippocampus), cultured astrocyte (cell lysis) and astrocyte conditioned medium. The kit works very well for cell lysis and brain tissues according to the instruction. For the cell medium, I suggest that users need modify the protocol a little bit, such as prolong the incubation time for samples in the wells for overnight. The kit has a good quality.
Detecting BDNF from brain tissues and primary cultures of astrocytes
Mouse brain tissues and primary cultures of astrocytes
Brain tissue: 1. collect brain tissue after treatment; 2. get tissue lysis and determine cell concentration; 3. load equal protein to each ELISA kit well;Cell: 1. collect cell lysis after treatment; 2. cell lysis and determine cell concentration; 3. load equal protein to each ELISA kit wellMedium: 1. collect cell medium after treatment; 2.load equal olume of medium to each ELISA kit well
For cell medium study, incubating the sample in the well overnight not 90 min
The kit can stably and easily detect the expression of BDNF in brain tissues and cell lysis. For the medium, users may need modify the procedure as shown in the tip
N/A
Good
Sensitivity may not be such high as suggested in the manual but good enough for measuring the above samples
It is a good kit with good quality. And the service of the company is very good. I recommended it.
Product Name: Mouse BDNF PicoKine ELISA KitSupplier Page