Licor
Streptavidin IRDye 800CW
926-32230
Info: View Product View Product Specs
Research aim: To measure changes in protein palmitoylation in response to various treatments. Cells were treated with a biotin analog in order to capture palmitoylated proteins. Cell lysates were subjected to SDS-PAGE and probed for biotin incorporation utilizing a streptavidin antibody. The antibody was chosen based on the ease of streptavidin for biotin capture (high affinity). The antibody is conjugated to a flurorpore, therefore, we can avoid the typical primary antibody followed by secondary incubations.
Western Blot
Total lysates - H4IIE liver hepatoma cells
1:2000 overnight at 4C@
1:3 Odyssey buffer:TBS with 0.1% Tween20
N/A
Odyssey Clx
Streptavidin antibody is detectable in a biotin dose dependent manner. Likewise, it does not show up in a loading control with no biotin label. I am really happy with the ease and crisp labeling pattern using this antibody.
Reliable and easy to use!
No major drawbacks
I would recommend this antibody to capture biotin labeling. It is easy to use, and optimize.