Consistent Detection Of iNOS In Mouse Peritoneal Macrophages

Pharmaceutical Sciences
University of Michigan
Research Fellow

Overall

Quality of Results

Ease-of-Optimization

What do these ratings mean?
Write a Review

Company:

Abcam

Product Name:

Anti-iNOS antibody

Catalog Number:

ab3523

Our projects require the testing of various inflammatory stimuli or agonists on peritoneal macrophages from mice that are either drug-treated or untreated. To confirm or test our hypotheses on what targets may have been modulated upon inflammatory stimuli and drug treatment, we utilize best practice western blot techniques to analyze and measure the protein/target of interest in our experiments.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse peritoneal macrophages (see results for more details)

Primary Incubation

1/500 of Ab in 5% milk in TBST, overnight, 4 deg C

Blocking Agent

Non-fat dry milk proteins in TBS

Secondary Incubation

1/5000 of HRP-Ab in 5% milk in TBST, 1 hour, room temperature

Tertiary Incubation

None

Detection

X-ray Development using ECL substrate and photofilm

Results Summary

The peritoneal macrophages were sorted using flow cytometry and the drug's inherent fluorescent signature. See - https://www.ncbi.nlm.nih.gov/pubmed/26109497. Post-sorting, the cells were plated for 24-48 hours before the agonists or inflammatory stimuli were applied. 8-24 hours post stimulus, cells were collected and lysed, measure for protein concentration and best practice western blot techniques were used as discussed above. As seen in the figure, the repeatable band production of iNOS in three different mice samples was easily verified and will be quantified via densitometry and normalized using B-actin as a loading control.

DOI or PMID #

10.1002/cyto.a.22706

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Consistent and repeatable

The Bad

None

The Bottom Line

A very effective Ab for detection of iNOS via western blot analysis of mouse peritoneal macrophages.

Share your experience with other scientists. Write a Review! »

Join the discussion