Use Of Desmin (Clone D33) Antibody From Dako On Myogenic Cell Culture

November 15, 2016

Pathology
BWH
Research Scientist

Overall

Quality of Results

Ease-of-Optimization

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Company:

Dako

Product Name:

Monoclonal Mouse Anti-Human Desmin (D33)

Catalog Number:

M076029-2

 

Browse Similar Products: Desmin Immunofluorescence Antibodies

This antibody was used to analyze the presence of myotubes in differentiated culture of mouse embryonic stem cells (mESCs). This monoclonal anti-Desmin (clone D33) antibody appears specific to myotubes and cardiomyocytes.

Experimental Design and Results Summary

Applications

Immunofluorescence

Sample

Mouse embryonic stem cells (mESCs), differentiated. Fixed Adherent culture.

Primary Incubation

1:150, overnight, at 4C in Blocking agent, on shaker

Blocking Agent

TBS, 1% FBS, 0.1% TritonX100, 30min, at RT

Secondary Incubation

1:500, AlexaFluor-488-conjugated anti-mouse IgG secondary (Molecular Probes), overnight, at 4C in Blocking agent, on shaker

Tertiary Incubation

N/A

Detection

Fluorescent microscope, FITC/GFP filter for AlexaFluor-488 detection

Results Summary

This antibody works very on fixed adherent culture containing myogenic cells. Specific to myotubes but also to cardiomyocytes.

DOI or PMID #

N/A

Additional Notes

N/A

Related Categories

Image Gallery

Summary

The Good

Specific signal to muscle cells by immunofluorescence

The Bad

Signal is moderately strong

The Bottom Line

Good antibody overall. Recommended for immunofluorescence study of skeletal myogenesis in mouse (myotubes). This antibody reacts with the intermediate filament Desmin. Signal is moderate. Will also recognize cardiomyocytes.

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