Expression of MCM4 in Human Aortic Smooth Muscle Cells

November 14, 2016

Pathology
University of Tennessee Health Science Centre
Postdoctoral Fellow

Overall

Quality of Results

Ease-of-Optimization

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Company:

Santa Cruz Biotechnology

Product Name:

Anti-MCM4 antibody

Catalog Number:

sc-28317

To check the MCM4 expression in Human Aortic Smooth Muscle Cells. Antibody detects the single MCM4 band in western blot.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Human Aortic Smooth Muscle Cells

Primary Incubation

1:500

Blocking Agent

10% Milk for 1 Hour

Secondary Incubation

1:4000

Tertiary Incubation

NA

Detection

ECL

Results Summary

Antibody detects single band at around 100 kDA in whole cell lysate

DOI or PMID #

NA

Additional Notes

NA

Related Categories

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Summary

The Good

Easy to optimise and produce single clear band

The Bad

Nothing

The Bottom Line

Antibody produces single clear band

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