Abcam
Anti-Phospholipase C gamma 1 antibody [2B1]
ab16955
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Phospholipase C gamma 1 protein levels were detected in femoral muscle of rats which were subject to 7 days of hind limb ischemia. For this, extracted proteins were resolved in SDS-PAGE, western transferred in nitrocellulose membrane and probed for Phospholipase C gamma 1 levels. Selection of antibody was based on previous experience with same antibody in human protein samples.
Western Blot
Whole rat tissue protein lysate
Overnight at 4 0C (5% BSA in TBS with 0.1% Tween 20; dilution 1:1000)
5 % Skimmed milk in TBS with 0.1% Tween 20 (for 1 hr at RT)
2 hr at RT (5% skimmed milk in TBS with 0.1% Tween 20; dilution 1:4000)
None
ECL
Antibody was highly specific for Phospholipase C gamma 1 as shown by only one band at a molecular weight of around 150 KDa. There was no none-specific signal detected.
Antibody is highly specific to Phospholipase C gamma 1
Excellent specificity of Abcam Phospholipase C gamma 1 antibody