Pacific Blue anti-human CD8 Antibody

Cancer Center
George Washington University
PhD Candidate

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

Pacific Blue™ Mouse Anti-Human CD8

Catalog Number:

558207

Our lab studies different ways of enhancing the killing capacity of HIV-specific effector cells, such as CD8 T cells. We perform in vitro killing assays where CTLs activity is tested in presence of HIV-1 infected cells. For these studies, human CD8 T cells are purified from donor peripheral blood, and good specific antibodies against human CD8 are needed to validate the purification quality, as well as to detect the cells in FACs-based assays.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Whole Human PBMC

Primary Incubation

1:200, 30 min at 4 degrees Celcius

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow cytometry

Results Summary

Good antibody with bright signal and high specificity

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Bright, good staining

The Bad

None

The Bottom Line

I recommend the use of this antibody.

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