Miltenyi Biotec
CD4 MicroBeads, human
130-045-101
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We use these beads to purify CD4+ T cells from human PBMCs. The protocol is very straight forward and gives consistent results.
Separation of human CD4 cells from PBMCs
Human PBMCs
Count cells, spin dow. Add 80uL of AutoMACS running buffer/10^7 cells and 20uL of beads/10^10 cells. Incubate at 4C for 15min. Wash cells and resuspend in 3ml. Load the cells on the LS column (also miltenyi), wash 3x with running buffer. Elute the cells using the plunger that comes with the columns.
Could use a lower amount of beads. I went down to 10uL/10^7 cells and still got a good yield of CD4 cells. You should titer the beads to find the optimal amount for you if you wish to save some beads.
Over 90% pure CD4 T cell population. Good yield. Very little CD4+ cells remaining in the flow through (CD4 negative fraction). Consistent results.
None
Consistent results. Easy, fast protocol.
Expensive
Great product to use for purification of human CD4 cells.