BioLegend
MojoSort™ Human CD4 Nanobeads
480013
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Our lab routinely uses beads for purification of different population of human cells. We tested this product from BioLegend in efforts to save some money on beads as these are cheaper than competitor's.
Purification of CD4 positive T cells
Human PBMCs
The protocol we tested was the alternative one using the LS colums (Miltenyi). According to the sales rep this protocol should work just as well as the protocol using the 15ml conical magnet. Resuspend PBMCs at 1*10^8/ml.. Add the nanobeads at 1:10 (10uL/100Ul of cells). Incubate on ice for 15min. Add buffer to 3ml. Put the cells in the LS column, wash 3x, elute cells.
Consult with the sales rep regarding the protocol. They later told us there are many optimization possibilities we could try.
Poor purity of the CD4+ cell fraction, only 25%.
None
Cheap
Does not work well. Would need to spend some time optimizing the protocol.
Would not recommend this product unless you have some time you are willing to spend optimizing it.
Product Name: MojoSort™ Human CD4 NanobeadsSupplier Page