Fluidigm
Anti-Human CD185-153Eu
3153020B
Browse Similar Products: CD185 Flow Cytometry Antibodies
Our group studies T cells in the blood of untreated chronic HIV+ individuals. We are constantly expanding and optimizing our T cell panels to measure various T cell subsets, activation, and inhibitory receptor phenotype. We have begun incorporating mass cytometry into our techniques to study T cell phenotypes in greater depth. Detecting CXCR5 expression on CD4+ T cells in the periphery has been a particular struggle for our lab by mass cytometry. While CXCR5 expression is high on B cells, it is dim on circulating CD4 T cells (pTFH cells). CXCR5-171 (clone 51505) or CXCR5 (clone RF8B2) we conjugated ourselves failed to detect the dim expression of CXCR5. CXCR5-153, however, works perfectly to detect both high and dim expression of CXCR5 on human PBMC.
Flow Cytometry
Human PBMC
1uL CXCR5-153 in 200uL PBS with 10^6 cells; 25 minutes; room temperature
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CyTOF (Fluidigm)
1uL CXCR5-153 worked great to stain human PBMC. The figure below shows how it can detect both dim and high CXCR5 on human CD4+ T cells and CD19+ B cells, respectively. We are very pleased that this clone is commercially available on a this metal in the middle of the panel where detection is optimal.
1uL CXCR5-153 works great to detect CXCR5 on CD4+ T cells and B cells
None.
CXCR5-153 is a great option for investigators who want to incorporate CXCR5 into their mass cytometry panel!