Biolegend
AF488 Anti-Human CD150 (SLAM)
306312
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Browse Similar Products: CD150 Flow Cytometry Antibodies
We optimized and incorporated SLAM-AF488 into a multi-color fluorescence cytometry panel focused on characterizing peripheral TFH cells. We measured SLAM on pTFH cells in human PBMC from HIV- and HIV+ subjects. We selected this product based on how it fit into an already-established fluorescence cytometry panel.
Flow Cytometry
Human PBMC
0.1uL SLAM-AF488 per 1 million cells in 200uL PBS; room temperature; 15 minute incubation
None
BD LSRFortessa
While optimizing SLAM-AF488 within a bigger panel we measured SLAM expression on B cells, CD4 T cells, and CD8 T cells at 24, 48, and 72 hours with and without SEB stimulation (1ug/mL). The results show that 0.1uL SLAM-AF488 works well to detect SLAM expression on all the cell types (as expected highest on B cells, but clear bimodal expression is visible on the T cells as evidenced in the dot plots).
Works great using only 0.1uL per sample; played well in a 10-color fluorescence panel
Can't think of anything bad.
Great option for measuring SLAM if your AF488/FITC channel is open!!