The Good
Reagent is very inexpensive for a fluorescent antibody, which is important because 2 ug (10 ul of the stock) is given per mouse. PE is very stable through tissue processing. Can stain later with either CD45.1 or CD45.2 ex vivo after i.v. stain in vivo.
The Bad
PE may not be the best fluor for this marker in certain experimental designs.
The Bottom Line
The technique for injecting antibody intravenously is useful for studying in vivo populations, but can get very expensive because micrograms of antibody are needed for each mouse. This reagent currently costs $0.35 per microgram (when purchasing 200 ug) making it probably the most cost effective reagent for this technique.