Sigma
Anti-β-Actin antibody
A5316
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The aim of this project is to study protein-protein interactions between components of the NF-kB pathway. In this experiment, specific components of the NF-kB pathway were over-expressed in HEK293 cells. The above antibody was used as loading control for different samples.
Western Blot
HEK293 cell lysate
10000 dilution in blocking buffer; overnight at 4 deg Celsius
5% BSA + 1x TBS-T
10000 dilution in blocking buffer; 1 hour at r/t
No
Amersham ECL Western Blotting Substrate
Sample loading was uniform
None
Specific signal
Good loading control antibody