Abcam
Anti-KIF3A antibody
ab11259
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The hedgehog signaling plays a critical role in embryonic development. It also controls adult tissue homeostasis and deregulation of this pathway results in basal cell carcinoma and medulloblastoma. A unique feature of this pathway is that it requires a tiny antenna like organelle called the primary cilium for signal transduction. My goal is to understand the molecular mechanism of hedgehog signal transduction at the primary cilium. This involves detecting protein-protein interactions of the pathway components, analysis of localization patterns during signal transduction, and transcriptional output measurements. In the present experiment, the ciliary localized kinesin protein, Kif3a, levels were tested by Western Blotting in 3T3 cells with a later goal to knockdown for functional assays.
Western Blot
NIH-3T3 cell lysate
1:1000 dilution in blocking buffer; 16 hr at 4 deg
5% milk powder in 1xTBS-T(0.1%)
1:10,000 dilution of Donkey anti-rabbit IgG-HRP in blocking buffer; 1 hr at room temp
None
ECL pico
Kif3a was expressed in 3T3 cells and the antibody from Abcam picked up a specific band at ~85 kDa marker size.
Specificity of the antibody
Recommended for Western Blotting of Kif3a. In addition, this antibody also works for immunofluorescence staining experiments.