EMD Millipore
Anti-Myc Tag Antibody, clone 9E10
05-419
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Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. I had discovered that class I and Class II ARF proteins and more specifically ARF1 can function as a tethering factor for Protein Kinase D at the Golgi complex. These conclusions were based on protein-protein interaction assays, colocalization experiments, and Golgi functional assays after knockdown down of Protein Kinase D in cells. In this experiment, over-expressed ARF1-Myc was immunoprecipitated from HEK293T cells, and blotted with a Myc-tag antibody from EMD Millipore
Western Blot
Whole cell extracts of HEK293T cells over-expressing ARF1-Myc
1:2000 dilution in blocking agent; 1 hr at room temp
5% Milk powder in 1xTBS-T(0.1%)
Goat-anti mouse IgG-HRP conjugated
None
ECL regular
This Myc tag antibody nicely detects over-expressed ARF1-Myc from HEK293T cells
Specific signal
One of the best antibodies in the market to detect Myc-tagged proteins by Western Blotting