EMD Millipore
Anti-GST Tag Antibody
06-332
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Browse Similar Products: GST Western Blot Antibodies
Protein Kinase D is family of Ser/Thr kinases that regulate multiple biological functions including cell migration, proliferation, apoptosis, transcriptional activation, angiogenesis, and Golgi organization and vesicle trafficking. In the present study, the molecular basis of Protein Kinase D recruitment to the Golgi complex was investigated. I had discovered that class I and Class II ARF proteins and more specifically ARF1 can function as a tethering factor for Protein Kinase D at the Golgi complex. These conclusions were based on protein-protein interaction assays, colocalization experiments, and Golgi functional assays after knockdown down of Protein Kinase D in cells. In this experiment, GST and GST-ARF1 were expressed and purified from bacteria. Relative levels of these proteins in a pull down experiment were determined by Western Blotting with GST antibody. This antibody was purchased by another graduate student in the lab for his specific application and recommended me to use it for my experimental purpose.
Western Blot
Recombinant proteins
1:2000 in blocking buffer; 1 hr at room temp
5% Milk powder in 1xTBS-T (0.1% Tween-20)
Goat-anti Rabbit-IgG-HRP conjugated
None
ECL regular
This GST antibody could detect both GST tag alone and GST-tagged proteins by Western Blotting.
Works as stated by the manufacturer.
Good antibody for detecting GST tagged proteins by Western Blotting