Novus Biologicals
HDAC9 antibody
NBP2-03993
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HDAC9 antibody was used to blot for HDAC9 in Human Coronary Artery Endothelial Cells
Western Blot
Human Coronary Artery Endothelial Cells (primary cell line)
1:1000 in 5% BSA in TBS-tween, overnight at 4 degrees celcius
5% Bovine Serum Albumen in TBS-tween 0.05%
1:15000, 1 hour at room temperature diluted in 5% Skim milk in TBS-tween (ThermoFisher Scientific 31460)
None
Imaged with enhanced chemiluminescence on a biorad Chemidoc touch, 10 second exposure
HDAC9 was detected in HCAECs as a very strong, clear single band at approx. 100kD
Antibody was very easy to use, and produced a very clear and specific result
Great antibody, will continue to use!