Anti-LMAN1 (Immunofluorescence) In HEK293 Cells

This antibody was used in a study investigating the effects of N-glycosylation of Matrix Metalloproteinase-9 (MMP9) by characterizing the intracellular organelle trafficking pattern of the N-glycoyslation mutants of MMP9. It has been published in Traffic in 2015 (Duellman et al., Functional Roles of N-Linked Glycosylation of Human Matrix Metalloproteinase 9, Traffic. 10, 1108-26). Anti-LMAN1 was used to characterize the staining pattern of the ERGIC. in HEK293 cells. Anti-LMAN1 stained 4% PFA fixed HEK293 cells and resulted in a weak but distinguishable signal and appeared to stain in a similar pattern as previously published immunofluorescent detection of the ERGIC by others. A dilution of 1:100 incubated for 3 hours was used, followed by secondary staining using Alexa-488. Using this antibody we concluded that N-glycosylation mutants of MMP9 were not trapped in the ERGIC.