Anti-Golgin 97 ICC Detection Of The Golgi Apparatus In HEK293 Cells

This antibody was used in a study investigating the effects of N-glycosylation of Matrix Metalloproteinase-9 (MMP9) by characterizing the intracellular organelle trafficking pattern of MMP9 N-glycoyslation mutants. It has been published in Traffic in 2015 (Duellman et al., Functional Roles of N-Linked Glycosylation of Human Matrix Metalloproteinase 9, Traffic. 10, 1108-26). Anti-Golgin 97 was used to characterize the staining pattern of the Golgi Apparatus and compared to a GFP molecular construct engineered to localize within the Golgi Apparatus. Anti-Golgin 97 stained the 4% PFA fixed HEK293 cells and resulted in a weak but distinguishable signal and appeared in a similar detection pattern as our Golgi-localizing GFP construct. A dilution of 1:100 incubated for 3 hours was used followed by secondary staining using Alexa-488.