Anti-Histone H2B Antibody Review Millipore

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Millipore

Product Name:

Anti-Histone H2B Antibody

Catalog Number:

07-371

We study epigenetics in embryonic stem cells. We use the H2b antibody as a loading control sometimes when analyzing histone modifications after different treatments in mouse ES cells.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Great antibody, clear signal, would use again.

Additional Notes

None

Related Categories

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Summary

The Good

Nice, clear signal

The Bad

Expensive

The Bottom Line

Great antibody, would use again!

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