Cell Signaling Technology
β-Catenin Antibody (Carboxy-terminal Antigen)
9587S
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We were using beta-catenin levels as a measure of wnt activity in mouse ES cells under different treatment conditions.
Western Blot
Mouse ES cells
1:1000
5% milk
1:3000 in 5% milk for one hour at RT
N/A
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
Pretty good antibody, some background but signal is clear.
None
Clear signal
Some background
Good antibody, would use again.