Biolegend
anti-Tubulin mouse mAb
627901
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Browse Similar Products: Tubulin Western Blot Antibodies
Anti-Tubulin mouse mAb #627901 from Biolegend is an excellent antibody to verify protein loading in western blot applications.
Western Blot
Tumor cell lysate
Overnight, at 4-8 degrees Celsius, with rocking, at 0.5 ug/mL in 5% milk/PBS/0.05% Tween 20
5% milk in PBS/0.05% Tween-20 (5% milk/PBS/Tw)
Goat anti-mouse IgG HRP-conjugated antibody # 7076 from Cell Siganaling; 1:3,000
None
SuperSignal West Pico Chemiluminescent Substrate (Thermo Scientific)
The mouse mAb #627901 from Biolegend is an excellent IgM antibody to detect alpha-tubulin in human tumor cell lysates. It is exceptionally sensitive and specific: there is no non-specific bands in our blots and it could be used at a relatively low concentrations ranging from 0.1 to 0.5 microgram/mL. This antibody could be used either following the detection of the protein of interest or simultaneously (provided that the secondary antibody would not recognize the primary antibody for the protein of interest).
The antibody can be reused up to 10-15 times if stored at -20oC after blotting.
The antibody is of exceptional quality
None detected
I would highly recommend the mouse mAb #627901 from Biolegend for detection of alpha-tubulin by western blotting.