Qiagen
QIAEX II Gel Extraction Kit (500)
20051
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This kit was used for isolating PCR fragments from agarose gels. This can also used for isolating DNA from poly acrylamide gels.
Gel purification and Cloning
PCR fragments
1. Excise DNA fragment from gel. 2. Weigh the gel and add 3 volume of QX13. Resuspend QIAEX II by vortexing for 30 s. Add QIAEX II to the sample according the data sheet.4. Incubate 50 degree fro 10 min. 5.Centrifuge the sample for 30 s and carefully remove supernatant with a pipet.6. Wash the pellet with 500 μl of Buffer QX1.7.Wash the pellet twice with 500 μl of Buffer PE.8.Air-dry the pellet for 10–15 min or until the pellet becomes white.9.To elute DNA, add 20 μl of 10 mM Tris·Cl, pH 8.5 or H2O and resuspend the pellet by vortexing. Incubate according to the table in the data sheet10. Centrifuge for 30 s. Carefully pipet the supernatant into a clean tube.11. Check the concentration and DNA is ready for the next application.
Carefully follow the protocol in the data sheet
I was able to purify PCR fragments using this kit
None
Easy protocol
Very useful kit for gel purification of DNA fragments
Product Name: QIAEX II Gel Extraction Kit (500)Supplier Page