Qiagen
QIAprep Spin Miniprep Kit (50)
27104
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I used Qiagen's QIAprep Spin Miniprep plasmid extraction kit for small scale pBAD DNA extraction from S. enterica cultures grown in LB for cloning experiments. This kit method based extraction gave me an yield of 6 to 7 microgram total with quality of 260:280 ratio 1.87.
Rapid plasmid DNA extraction from bacterial cultures for cloning experiments
Overnight grown 3 ml S. enterica cultures in LB broth
S. enterica cultures grown overnight in the LB broth (3 ml) were pelleted by centrifugation at 10000 rpm at room temperature for 4 minutes. Then these pellets were used in the extraction using miniprep kit's protocol with lysis, neutralisation, washing buffers provided with the kit. Finally, plasmid DNA was eluted in 50 microliter of nuclease free molecular biology grade water.
If you want to increase the concentration of total plasmid DNA, you elute with smaller volume like 20 or 30 microliter volumes.
Finally I got good yield using this kit. I got around 130 to 140 ng/microliter yield with 260:280 ratio of 1.87 (checked by Nanodrop ND1000 spectrophotometer.
None
Easy to follow and rapid extraction of small quantity of plasmid DNA
Recommended if one intended to get rapid plasmid DNA from the cultures.
Product Name: QIAprep Spin Miniprep Kit (50)Supplier Page