The Antibody Identifies CD45+ Cells In The Colon Well

July 27, 2016

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

BD Biosciences

Product Name:

BUV737 Mouse Anti-Mouse CD45.2

Catalog Number:

564880

We wanted to study the immune cells in the intestine in a diseased vs healthy state, so we used this antibody to identify CD45+ cells by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of colon cells

Primary Incubation

1:100

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody clearly distinguishes CD45+ cells in the colon

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

The antibody stains CD45+ cells well

The Bad

None to mention

The Bottom Line

The antibody is good for identifying CD45+ immune cells in the colon

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