The Antibody Stains MHCII+ Cells Very Clearly

July 19, 2016

Pathology
Stanford
Graduate Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

eBioscience

Product Name:

Anti-Mouse MHC Class II (I-A/I-E) APC-eFluor® 780

Catalog Number:

47-5321-82

We wanted to study the immune cells in the intestine in a diseased vs healthy state, so we used this antibody to identify MHCII+ cells by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Single-cell suspension of colon cells

Primary Incubation

1:100

Blocking Agent

FcR Block

Secondary Incubation

None

Tertiary Incubation

None

Detection

BD LSR II flow cytometer

Results Summary

The antibody clearly distinguishes MHCII+ cells in the mouse colon.

Additional Notes

https://www.biocompare.com/Search-Antibodies/?search=MHC%2520Class%2520II&said=0&soids=1529

Related Categories

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Summary

The Good

Antibody stains MHCII+ cells very well

The Bad

None to mention

The Bottom Line

The antibody is great for identifying MHCII+ cell populations.

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