Extremely Reliable Anti-HA Antibody

July 06, 2016

Protein Degradation
WGL
PI

Overall

Quality of Results

Ease-of-Optimization

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Company:

Roche

Product Name:

rat Anti-HA High Affinity

Catalog Number:

11867431001

Browse Similar Products: Anti-HA Western Blot Antibodies

We analyzed samples of crude Arabidopsis thaliana (mouse-ear cress) protein extract from seedlings or later developmental stages according to their protein content. HA tagged proteins in Arabidopsis tissue extracts and in samples containing recombinant protein can be easily detected using this antibody.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Arabidopsis seedlings or leaves

Primary Incubation

1 to 1000, overnight

Blocking Agent

5% milk TBST

Secondary Incubation

1 to 2500

Tertiary Incubation

n.a.

Detection

tetrazolium

Results Summary

HA-tagged proteins can be easily detected using this antibody, regardless whether recombinantly expressed in bacteria, or transiently or stably transformed and expressed in plants.

Additional Notes

none

Related Categories

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Summary

The Good

Very good clear signal and reproducibility

The Bad

none

The Bottom Line

Extremely reliable anti-HA antibody

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