Biolegend
PE/Cy7 Anti-mouse TCR DO11.10
118516
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The aim of the experiment was to assess DO11.10 T cell differentiation into Th1 or Th2 subset after co-incubation with BALB/c splenocytes, ova antigen, and LPS. This antibody was necessary to gate on the specific T cell population in the mixed population.
Flow Cytometry
Mouse lymphocytes
≤0.5 µg per million cells in 100 µl volume (manufacturer's recommendation) 30min. on ice.
None
Fortessa
The TCR+ population is easily gated on using this antibody.
Bright
Cost
Good antibody and would recommend for experiments to detect the DO11.10 T cell population.