AKTA Chromatography

National Institute of Nutrition
Biochemistry
Senior research fellow

Overall

Performance

Ease-of-Optimization

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Company:

GE Life Sciences

Product Name:

ÄKTA prime plus

Catalog Number:

11-0013-13

Protein purification involves isolating proteins from the source such as bacteria, yeast, or mammalian cells in culture. This is based on differences in their physical properties. Purified proteins are required for many experimental applications, including structural studies and in vitro biochemical assays.

Experimental Design and Results Summary

Application

Isolate the purified various types of proteins from tissues.

Starting Material

Goat lens total proteins

Protocol Overview

After collecting the lens, extracted the total protein fraction in physiological buffer. This protein solution is loaded in the columns. Then set the parameters according to our requirement.

Tips

Check the back pressure constantly.

Results Summary

Found purified various alpha crystalline proteins in goat lens

Features Summary

User friendly software.

Additional Notes

None

Related Categories

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Summary

The Good

Lamp durability and software.

The Bad

Expensive

The Bottom Line

Essential for protein purification labs, if cost is ok.

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