Very Good Pacific Blue Conjugated Anti-Human CD3 Antibody

June 16, 2016

Dept of biomedical Sciences
TTUHSC El Paso
Post doc

Overall

Quality of Results

Ease-of-Optimization

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Company:

Biolegend

Product Name:

Pacific Blue™ anti-human CD3 Antibody

Catalog Number:

300330

Human PBMCs were stained with either Isotype or Pacific Blue™ anti-human CD3 antibody. Cells were washed after 30 min and analyzed by flow cytometry.

Experimental Design and Results Summary

Applications

Flow Cytometry

Sample

Human PBMCs

Primary Incubation

30 min

Blocking Agent

None

Secondary Incubation

None

Tertiary Incubation

None

Detection

Flow Cytometry on PB Channel

Results Summary

Clear CD3 positive population indicates the specificity of this antibody

Additional Notes

None

Related Categories

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Summary

The Good

Good Specificity

The Bad

None

The Bottom Line

Good antibody for detecting human CD3 expression

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