Excellent for Tyrosine Hydroxylase IHQ

June 09, 2016

Pathology
Federal University of Paraná
Student

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma

Product Name:

Anti-Tyrosine Hydroxylase

Catalog Number:

T2928

Browse Similar Products: Tyrosine Hydroxylase Antibodies

We were evaluating survival of dopaminergic neurons in an animal model of Parkinson's Disease.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Tissue fixed with 4% PFA

Primary Incubation

1:750, Over night, 4˚C, incubated in 5% BSA

Blocking Agent

5% BSA in PBS

Secondary Incubation

Secondary antibody HRP-conjugated (Don't know the concentration - it was detected using a Kit)

Tertiary Incubation

Not necessary

Detection

Diaminobenzidine

Results Summary

The antibody reacts perfectly, it does not show any cross-reaction and gives a really beautiful result.

Additional Notes

None

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Summary

The Good

It does not give any cross-reaction.

The Bad

Nothing

The Bottom Line

It is one of the best antibodies I have ever tested

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