Sigma Monoclonal ANTI-FLAG® M2 antibody produced in mouse review

June 08, 2016

Genetics
Yale
Tech

Overall

Quality of Results

Ease-of-Optimization

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Company:

Sigma Aldrich

Product Name:

Monoclonal ANTI-FLAG® M2 antibody produced in mouse

Catalog Number:

F1804-200UG

Browse Similar Products: M2 Western Blot Antibodies

We do a lot of knockouts and knock-ins using crispr-cas9 technology in order to study epigenetics in mouse ES cells. We use the Flag-tag in knock-in constructs.

Experimental Design and Results Summary

Applications

Western Blot

Sample

Mouse ES cells

Primary Incubation

1:1000 4C overnight

Blocking Agent

5% milk

Secondary Incubation

1:3000 in 5% milk for one hour at RT

Tertiary Incubation

N/A

Detection

Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad

Results Summary

Great antibody, minimal background, clear band.

Additional Notes

None

Related Categories

Image Gallery

Summary

The Good

Strong clear band, strong antibody

The Bad

N/A

The Bottom Line

Great antibody! A staple in our lab!

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