Abcam
Anti-Histone H3 antibody - Nuclear Loading Control and ChIP Grade
ab1791
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Browse Similar Products: Anti-Histone H3 Western Blot Antibodies
We study epigenetics in embryonic stem cells. We use the H3 antibody as a loading control when analyzing histone modifications after different treatments in mouse ES cells.
Western Blot
Mouse ES cells
1:50000 2 hours RT on shaker
5% milk
1:3000 in 5% milk for one hour at RT
N/A
Exposure with SuperSignal™ West Femto Maximum Sensitivity Substrate, imaged with apparatus from Bio-Rad
This antibody is very, very strong and tends to become overexposed easily in SuperSignal™ West Femto Maximum Sensitivity Substrate if used at the dilution recommended in the manual (with mouse ES cell lysates). To avoid overexposure, I try to use at 1:50000 dilution when working with mouse ES cell samples.
None
Antibody is very strong, can use very little and still pick up signal
If too much is used, analyzing resulting image becomes difficult
Use at very low concentration and it should work alright!