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FITC anti-human CD34 Antibody
343504
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Human synovial cells yielded by enzymatic digestion were stained with anti-CD34 to identify cell populations present in synovium.
Flow Cytometry
Human synovial cells
30 minute incubation at 4oC. Live/dead stain.
No specific blocking step. 2% FCS in staining buffer.
30 minute incubation at 4oC. Antibody cocktail used at this point containing antibody at 10ug/ml.
None
Mo Flow Astrios EQ 488 excitation 540/40 bandpass filter for emission
Antibody stained positive population however the staining was not very bright and as a result the population was visible as a shoulder on the negative peak
Antibody does stain positive cells
Staining is not very bright
Antibody does stain positive cells however the resolution is not great due to the dim intensity of the population