Cell Signaling Technology
IL-1β (D3U3E) Rabbit mAb
12703
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Mouse macrophage cells were knockdown for specific protein using its specific siRNA, growth arrested and treated with growth factor for specific time point. Cells were lysed in RIPA buffer, protein extract were made, and using western blot tested for expression levels of IL-1β using its specific antibodies. Cell Signaling Technology antibody was selected because of picture provided in antibody flyer.
Western Blot
Mouse macrophage cells extract
Dilution (1:1000) in 5% skimmed milk prepared in TBST, overnight incubation at 4 degrees Celsius
5% skimmed milk prepared in TBST
Dilution (1:4000) in 5% skimmed milk prepared in TBST, 1hr at RT
None
Enhanced chemiluminiscent detection in X-Ray film
Poor detection of IL-1ß was observed with antibody. However, there were less non-specific bands so; it can be graded as average antibody for IL-1ß in mouse macrophage cells.
Gives IL-1ß band
Average antibody